新研博美荧光基团选择指南

发布时间：

2025-08-14

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荧光染料是指吸收某一波长的光波后能发射出另一波长大于吸收光的光波的物质。它们大多是含有苯环或杂环并带有共轭双键的化合物。荧光染料可以单独使用，也可以组合成复合荧光染料使用。有机荧光材料在生物成像方面是一类应用性很强的发光材料，在近几年飞速发展的研究进展中，有机荧光染料已被广泛应用于标记蛋白质、DNA检测和环境检测等领域。有机荧光材料基于单光子激发过程，常以能量较高，波长较短的光激发，发射光具有能量较低，波长较长的特点。高选择性、高灵敏度、高荧光量子产率等都是有机荧光化合物的优点。

荧光染料的种类繁多，其中罗丹明和荧光素是应用广泛的两种荧光染料。常见的荧光染料还有AF类，Cy类，BDP类，硅基罗丹明类，ATTO类，四甲基罗丹明类，ICG类以及离子探针等等。每款荧光染料中又包含很多荧光基团，荧光基团使研究人员能够在高内涵分析、显微镜和流式细胞仪平台中突破其多色实验的限制。我们的荧光染料可与抗体、链霉亲和素、肽、蛋白质、示踪剂和扩增底物偶联，并针对细胞标记和检测进行了优化。它们也可用于标记试剂和试剂盒，使研究人员能够自己标记生物分子，用于各种应用以及探测生物结构、功能和相互作用。浏览下表，您可以找到每个荧光基团的信息。

荧光分子	颜色	激发波长	发射波长
Alexa Fluor 350	Blue	346 nm	442 nm
Alexa Fluor 405	Blue	402 nm	421 nm
Pacific Blue	Blue	410 nm	455 nm
DAPI	Blue	340 nm	488 nm
AMCA	Blue	350 nm	450 nm
Cascade Blue	Blue	400 nm	420 nm
EviTag(TM) quantum dots - Lake Placid Blue (490 nm)	Blue	<400 nm	490 nm
Fluoroblue	Blue	350 nm	450 nm
Image-Blue	Blue	380 nm	465 nm
Marina Blue	Blue	365 nm	460 nm
Pacific Blue	Blue	410 nm	455 nm
Cy2	Green	491 nm	509 nm
Alexa Fluor 430	Green	434 nm	539 nm
Alexa Fluor 488	Green	495 nm	519 nm
Alexa Fluor 488 FluoroNanogold	Green	495 nm	519 nm
Alexa Fluor 500	Green	503 nm	525 nm
Alexa Fluor 514	Green	518 nm	540 nm
FITC	Green	490 nm	520 nm
Fluorescein	Green	494 nm	518 nm
HNP	Green	290 nm	509 nm
5-FAM	Green	494 nm	522 nm
6-FAM	Green	492 nm	517 nm
BODIPY FL	Green	505 nm	513 nm
CMNB-caged Fluorescein	Green	494 nm	518 nm
Cyanine 2	Green	492 nm	510 nm
DTAF	Green	495 nm	520 nm
DTAF/FITC	Green	495 nm	520 nm
dUTP-Biotin and Avidin FITC	Green	495 nm	520 nm
dUTP-FITC	Green	495 nm	520 nm
EviTag(TM) quantum dots - Adirondack Green (520 nm)	Green	<400 nm	520 nm
EviTag(TM) quantum dots - Catskill Green (540 nm)	Green	<400 nm	540 nm
FITC (Fluorescein)	Green	495 nm	520 nm
Fluorescein-5-EX, succinimidyl ester (FSE)	Green	495 nm	520 nm
Hi FITC	Green	495 nm	520 nm
JOE	Green	520 nm	548 nm
MFP488	Green	501 nm	523 nm
Oregon Green 488	Green	496 nm	524 nm
Oregon Green 514	Green	511 nm	530 nm
Qdot 525	Green	488 nm	515 nm
TET	Green	521 nm	536 nm
CAL Fluor(R) Gold 540	Green	522 nm	544 nm
NL493	Green	493 nm	514 nm
GFP	Green	395/475 nm	509 nm
Cy3	Yellow	548 nm	562 nm
Cy3B	Yellow	558 nm	572 nm
Alexa Fluor 532	Yellow	531 nm	554 nm
Alexa Fluor 546	Yellow	556 nm	573 nm
Alexa Fluor 555	Yellow	555 nm	565 nm
R-Phycoerythrin	Yellow	480, 546, 565 nm	578 nm
B-Phycoerythrin	Yellow	546, 565 nm	575 nm
HNP/Fast Red TR	Yellow	290 nm	562 nm
5-TAMRA	Yellow	546 nm	574 nm
Atto 550	Yellow	554 nm	576 nm
CAL Fluor(R) Orange 560	Yellow	538 nm	559 nm
Cyanine 3	Yellow	550 nm	570 nm
DyLight 547	Yellow	547 nm	574 nm
EviTag(TM) quantum dots - Birch Yellow (580 nm)	Yellow	<400 nm	580 nm
EviTag(TM) quantum dots - Hops Yellow (560 nm)	Yellow	<400 nm	560 nm
HEX	Yellow	535 nm	556 nm
NED	Yellow	546 nm	575 nm
Qdot 565	Yellow	<500 nm	565 nm
Quasar(R) 570	Yellow	548 nm	566 nm
R-Phycoerythrin (R-PE)	Yellow	488 nm	575 nm
TRITC (Rhodamine)	Yellow	550 nm	570 nm
VIC	Yellow	538 nm	554 nm
NL557	Yellow	557 nm	574 nm
Phycoerythrin	Yellow	480-566 nm	575 nm
Cy3.5	Orange	581 nm	596 nm
Alexa Fluor 568	Orange	578 nm	603 nm
ROX	Orange	575 nm	602 nm
DyeMer 488/605	Orange	502 nm	609 nm
DyeMer 488/615	Orange	502 nm	615 nm
CAL Fluor(R) Red 590	Orange	569 nm	591 nm
CAL Fluor(R) Red 610	Orange	569 nm	610 nm
Cyanine 3.5	Orange	581 nm	596 nm
DyeMer 488/605	Orange	488 nm	605 nm
DyeMer 488/615	Orange	488 nm	615 nm
EviTag(TM) quantum dots - Fort Orange (600 nm)	Orange	<400 nm	600 nm
Lissamine Rhodamine	Orange	570 nm	590 nm
MFP555	Orange	560 nm	585 nm
Qdot 585	Orange	532 nm	585 nm
Qdot 605	Orange	<600 nm	605 nm
ROX	Orange	575 nm	602 nm
R-Phycoerythrin-Texas Red (PE-Texas Red, ECD)	Orange	480-565 nm	615 nm
Rhodamine Red-X	Orange	570 nm	590 nm
TMR red	Red	540 (520-560) nm	580 (570-620) nm
Cy5	Red	646 nm	664 nm
Cy5.5	Red	673 nm	692 nm
CypHer5E	Red	644 nm	663 nm
Alexa Fluor 594	Red	590 nm	617 nm
Alexa Fluor 594 FluoroNanogold	Red	590 nm	617 nm
Alexa Fluor 610	Red	612 nm	628 nm
Alexa Fluor 610–R-PE	Red	496, 546, 565 nm	630 nm
Alexa Fluor 633	Red	632 nm	647 nm
Alexa Fluor 635	Red	633 nm	647 nm
Alexa Fluor 647	Red	650 nm	668 nm
Alexa Fluor 647–R-phycoerythrin	Red	496, 546, 565 nm	668 nm
Alexa Fluor 660	Red	663 nm	690 nm
Texas Red	Red	596 nm	620 nm
DyeMer 488/630	Red	502 nm	630 nm
Allophycocyanin	Red	650 nm	660 nm
PI	Red	535 nm	617 nm
Allophycocyanin (APC)	Red	650 nm	660 nm
Allophycocyanin-Cyanine 5	Red	633 nm	670 nm
Allophycocyanin-Cyanine 5.5	Red	633 nm	690 nm
Atto 647	Red	645 nm	669 nm
CAL Fluor(R) Red 635	Red	618 nm	637 nm
Cyanine 5	Red	650 nm	670 nm
DyLight 647	Red	647 nm	673 nm
DyeMer 488/630	Red	488 nm	630 nm
Dyomics 647	Red	660 nm	673 nm
Lightcycler Red- 640	Red	625 nm	640 nm
PBXL-1	Red	545 nm	666 nm
PBXL-3	Red	614 nm	662 nm
PerCP (Peridinin Chlorophyll)	Red	490 nm	675 nm
PerCP-Cy5.5	Red	488 nm	695 nm
Qdot 655	Red	<620 nm	655 nm
Quantum Red	Red	488 nm	660 nm
Quasar(R) 670	Red	647 nm	667 nm
R-Phycocyanin (R-PC)	Red	614 nm	647 nm
R-Phycoerythrin-Cyanine 5 (Cy-Chrome, PC5, Tri-Color, Spectral Red)	Red	488 nm	660 nm
Sulforhodamine	Red	585 nm	620 nm
Texas Red	Red	596 nm	620 nm
Cyanine Q (Cyanine 5.18)	Red	488 nm	670 nm
NL637	Red	637 nm	658 nm
Allophycocyanin	Red	620-650 nm	660-670 nm
CytoCy5S	Red	628 nm	638 nm
Alexa Fluor 680	Far-Red	679 nm	702 nm
Alexa Fluor 680–Allophycocyanin	Far-Red	650 nm	702 nm
Alexa Fluor 680-R-phycoerythrin	Far-Red	555 nm	700 nm
Alexa Fluor 700	Far-Red	702 nm	723 nm
Alexa Fluor 700–Allophycocyanin	Far-Red	650 nm	723 nm
Alexa Fluor 750	Far-Red	749 nm	775 nm
Alexa Fluor 750–Allophycocyanin	Far-Red	650 nm	775 nm
Allophycocyanin-Cyanine 7	Far-Red	633 nm	760 nm
Cyanine 5.5	Far-Red	678 nm	703 nm
Cyanine 7	Far-Red	710 nm	805 nm
IRDYE700DX	Far-Red	689 nm	700 nm
IRDYE800	Far-Red	778 nm	795 nm
IRDYE800CW	Far-Red	774 nm	800 nm
NIRD700	Far-Red	682 nm	701 nm
Qdot 705	Far-Red	<700 nm	705 nm
Qdot 800	Far-Red	<600 nm	800 nm
R-Phycoerythrin-Cyanine 5.5	Far-Red	488 nm	703 nm
R-Phycoerythrin-Cyanine 7 (PC7)	Far-Red	488 nm	805 nm